Knowledge of the fasting plasma glucose of healthy women may assist in the setting of treatment targets for women with hyperglycaemia in pregnancy (HIP). This study examines the pregnancy glucose tolerance test results of 3344 women without HIP collected over a three‐year period. The median fasting plasma glucose was 4.4 mmol/L with an interquartile range of 4.2–4.6 mmol/L and a 5th to 95th centile of 3.8–4.9 mmol/L. As the diagnostic fasting glucose level for HIP is ≥5.1 mmol/L, these data support a treatment target of ≤5.0 mmol/L.